AG-DNA Micro Column provides
for the rapid isolation of total DNA (e.g., genomic,
mitochondrial, viral) from a variety of biological sample sources. This
product has been optimized for maximal recovery of ultra-pure DNA without RNA
contamination and is compatible with whole blood (fresh or stored), serum,
plasma, buffy coat, buccal cells, cells from culture, and many biological
Up to 5 µg total
DNA is eluted into ≥10 µl (6 µl minimum) DNA Elution Buffer or water. Human
whole blood will typically yield 1.5-3.5 µg DNA per 50 µl blood sampled.
Mammalian tissues already homogenized yield: 1-3 µg DNA per mg skeletal,
heart, and brain tissues and 3-5 µg DNA per mg liver, kidney and lung
DNA Size Limits
recovering genomic DNA up to and above 40 kb. In most instances,
mitochondrial DNA and viral DNA (if present) will also be recovered.
plasma, or serum from humans, mice, rats, etc. Also, cells from culture,
buccal cells, as well as a variety of biological liquids are effectively
processed using this kit. Tissue already digested with Proteinase K or
mechanically homogenized can also be processed.
is eluted with DNA Elution Buffer or water. DNA is especially well suited for
PCR and other downstream applications. A260/A280>1.8
The AG 96 Well DNA Binding Column is designed for
high-throughput purification of DNA from 1-5 ml of cultured bacterial cells per
well using an efficient 96 well filter plate and 96 well binding
plate system. A modified alkaline lysis method and RNase treatment are used to
obtain clear cell lysate with minimal genomic DNA and RNA contaminants. Typical
yields are 20-35 μg for high-copy number plasmid or 3-10 μg for low-copy number
plasmid from 4 ml of cultured bacterial cells. DNA phenol extraction or alcohol
precipitation is not required and the entire procedure can be completed within
25 minutes. The purified plasmid DNA is ready for use in restriction enzyme
digestion, ligation, PCR, and sequencing reactions.
Purify 96 wells of pure genomic DNA
in 45 minutes!
Sample Volume: up to 200 µl of whole blood,
plasma, serum, body fluids and up to 5 x 106 lymphocytes or cultured
cells per well
Yield: up to 6 μg of genomic DNA from
200 µl of whole blood per well