Angen Biotech Co.,LtdContact us
Angen Biotech Co.,Ltd
[China]
AG-DNA Micro Column provides for the rapid isolation of total DNA (e.g., genomic, mitochondrial, viral) from a variety of biological sample sources. This product has been optimized for maximal recovery of ultra-pure DNA without RNA contamination and is compatible with whole blood (fresh or stored), serum, plasma, buffy coat, buccal cells, cells from culture, and many biological liquid samples.
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DNA Recovery |
Up to 5 µg total DNA is eluted into ≥10 µl (6 µl minimum) DNA Elution Buffer or water. Human whole blood will typically yield 1.5-3.5 µg DNA per 50 µl blood sampled. Mammalian tissues already homogenized yield: 1-3 µg DNA per mg skeletal, heart, and brain tissues and 3-5 µg DNA per mg liver, kidney and lung tissues. |
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Equipment |
microcentrifuge, vortex |
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DNA Size Limits |
Capable of recovering genomic DNA up to and above 40 kb. In most instances, mitochondrial DNA and viral DNA (if present) will also be recovered. |
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Sample Sources |
Whole blood, plasma, or serum from humans, mice, rats, etc. Also, cells from culture, buccal cells, as well as a variety of biological liquids are effectively processed using this kit. Tissue already digested with Proteinase K or mechanically homogenized can also be processed. |
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DNA Purity |
High-quality DNA is eluted with DNA Elution Buffer or water. DNA is especially well suited for PCR and other downstream applications. A260/A280>1.8 |
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Product Detergent Tolerance |
≤5% Triton X-100, ≤5% Tween-20, ≤5% Sarkosyl, ≤0.1% SDS. |
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Workflow Overview |
Unique lysis buffer system omits the need for Proteinase K digestion for biological fluids and cell culture samples. |
96 Well DNA Binding Column #D80011
The AG 96 Well DNA Binding Column is designed for high-throughput purification of DNA from 1-5 ml of cultured bacterial cells per well using an efficient 96 well filter plate and 96 well binding plate system. A modified alkaline lysis method and RNase treatment are used to obtain clear cell lysate with minimal genomic DNA and RNA contaminants. Typical yields are 20-35 μg for high-copy number plasmid or 3-10 μg for low-copy number plasmid from 4 ml of cultured bacterial cells. DNA phenol extraction or alcohol precipitation is not required and the entire procedure can be completed within 25 minutes. The purified plasmid DNA is ready for use in restriction enzyme digestion, ligation, PCR, and sequencing reactions.
Advantages
Purify 96 wells of pure genomic DNA in 45 minutes!
Sample Volume: up to 200 µl of whole blood, plasma, serum, body fluids and up to 5 x 106 lymphocytes or cultured cells per well
Yield: up to 6 μg of genomic DNA from 200 µl of whole blood per well
Format: 96 Well Binding Plates
Elution Volume: 200 ~ 400 µl
Centrifuge or vacuum manifold protocol
Storage: dry at room temperature (15-25ºC)
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